Annual Single Cell Analysis Investigators Meeting 2015

By Single Cell Analysis Program

Date and time

April 20, 2015 · 8am - April 21, 2015 · 12pm EDT

Location

Natcher Conference Center

Kirschstein Auditorium 9000 Rockville Pike Bethesda, MD 20892

Description

ABOUT THE MEETING

The Single Cell Analysis Program (SCAP), supported by the National Institutes of Health (NIH) Common Fund, will host its 3rd Annual Investigators Meeting on April 20-21, 2015, at the Natcher Conference Center on the NIH campus in Bethesda, Maryland. The purpose of SCAP is to accelerate the discovery, development, and translation of cross-cutting, innovative approaches to analyzing the heterogeneity of biologically relevant populations of cells in situ.


MEETING OBJECTIVES

  • Convene the funded SCAP investigative teams to update the community on their research and consider current conceptual, technical, and methodological challenges in single cell analysis.
  • Determine major biomedical research opportunities that can be addressed by the Common Fund rather than individual NIH Institutes or Centers.
  • Discuss how relevant groundbreaking technologies and approaches in SCA can be disseminated to the research community effectively in the near future.

AGENDA DATES AND TIMES

Download the agenda booklet
Download breakout session descriptions
Download poster abstracts

MONDAY, APRIL 20, 2015

7:30 a.m. Registration and Check-In 8:00 a.m.

Welcome & Opening Remarks

  • Thomas Insel, M.D., Director of the National Institute of Mental Health
  • Roderic Pettigrew, Ph.D., M.D., Director of the National Institute of Biomedical Imaging and Bioengineering
8:15 a.m. Presentation of Awards for the NIH “Follow that Cell” Challenge

8:30 a.m.

Keynote Address
“A new focus on the cell: The Allen Institute for Cell Science”
Alan “Rick” Horwitz, Ph.D., Allen Institute for Cell Science

9:00 a.m.

“Expansion microscopy: Towards imaging at arbitrary resolution, scale, and multiplexing”
Edward Boyden, Ph.D., MIT Media Lab and McGovern Institute, MIT

9:20 a.m.

“Towards the living connectome: Imaging of individual neurons in intact, developing embryos”
Daniel Colón-Ramos, Ph.D., Department of Cell Biology, Yale University School of Medicine

9:40 a.m. Break 10:00 a.m. Lightning Talks 11:00 a.m. Break 11:20 a.m.

Keynote Address
“Probing stem cell biology with single-cell gene expression”
Stuart Orkin, M.D., Dana Farber Cancer Institute, Boston Children's Hospital, Harvard Medical School

11:50 a.m.

“Single-cell dissection of transcription factor expression heterogeneity in a lymphocyte developmental gene network”
Ellen Rothenberg, Ph.D., Division of Biology & Biological Engineering, California Institute of Technology

12:10 p.m.

“Detection of cell-type specific effects of pathway manipulation in neural cells”
Tracy Young-Pearse, Ph.D., Brigham and Women's Hospital; Harvard Medical School

12:30 p.m. Lunch on Your Own 1:15 p.m. Poster Session 3:15 p.m.

Keynote Address
“Dynamic functional heterogeneity of primary immune cells revealed by single-cell transcriptomics”
Hongkun Park, Ph.D., Department of Chemistry and Chemical Biology, Harvard University

3:45 p.m.

“Single cell transcriptomics analysis of neurons and cardiomyocytes from live human tissue”
Mugdha Khaladkar, Ph.D., Perelman School of Medicine, University of Pennsylvania
Jennifer Singh, Ph.D., Perelman School of Medicine, University of Pennsylvania

4:05 p.m.

“Dynamic single-cell analysis and interactions in cancer, allergy and senescence”
Tania Konry, Ph.D., Department of Pharmaceutical Sciences, Northeastern University

4:25 p.m.

“Cross-scale integrin regulation organizes ECM and tissue topology”
Scott Holley, Ph.D., Department of Molecular, Cellular and Developmental Biology, Yale University

4:45 p.m. Break 5:00 p.m. Breakout Sessions 7:00 p.m. Day 1 General Meeting Adjourns

TUESDAY, APRIL 21, 2015

7:30 a.m. Registration and Check-In 8:00 a.m.

Keynote Address
“Targeted proteomics with single-cell and sub-cellular resolution”
Amy Herr, Ph.D., Department of Bioengineering, University of California, Berkeley

8:30 a.m.

“Single-cell metabolic imaging using molecular fingerprinting”
Ji-Xin Cheng, Ph.D., Weldon School of Biomedical Engineering, Purdue University

8:50 a.m.

“Single-cell microsampling mass spectrometry for elucidating cell heterogeneity in the developing embryo”
Peter Nemes, Ph.D., Department of Chemistry, George Washington University

9:10 a.m. Break 9:30 a.m.

Keynote Address
“Towards a human cell atlas”
Aviv Regev, Ph.D., Department of Biology, MIT; Broad Institute of MIT & Harvard

10:00 a.m.

“Single-cell SNP FISH reveals epigenetic mosaicism in genomic imprinting mutants”
Arjun Raj, Ph.D., Department of Bioengineering, University of Pennsylvania

10:20 a.m.

“Characterizing neuronal subtypes in human adult cortex with single-nucleus transcriptome sequencing”
Kun Zhang, Ph.D., Bioengineering Department, University of California, San Diego

10:40 a.m.

“Transcription profiling in situ by seqFISH”
Long Cai, Ph.D., Division of Biology and Biological Engineering, California Institute of Technology

11:00 a.m. Breakout Session Reports 11:55 a.m. Wrap-Up and Summary 12:00 p.m. Meeting Adjourns

(A separate workshop for the SCAP-Transcriptome U01 groups and other invited grantees will be held following the PI meeting.)

DIRECTIONS & NIH VISITOR INFO

NIH Natcher Conference Center (Building 45)
9000 Rockville Pike, Bethesda, Maryland (see map)- Kirschstein Auditorium
Medical Center Metro Station (Red Line)

AIRPORTS/METRO

Reagan National Airport (DCA) is the closest airport and is connected to the Washington Metro. Dulles International Airport (IAD) and Baltimore Washington International Airport (BWI) are approximately 45 minutes from the NIH campus but are not accessible through the Washington Metro.

The NIH campus (located at the Medical Center Station) is accessible from the recommended Bethesda Hyatt (located at the Bethesda Station) via the Red Line of the Washington Metro.

NIH VISITOR INFORMATION

The NIH campus requires a valid, current, photo ID for entry. Visitor passes must be worn at all times. If you leave campus and return at a later time, you will be required to go through security again upon re-entry. If you choose to travel to the NIH by car, please note that pay parking is available but space is extremely limited. All vehicles and passengers must be screened at the Gateway Visitor’s entrance. Please allow adequate time for security screening.

Visit the NIH visitor’s web page for more information.

FOOD & BEVERAGES

Food and beverages must be purchased. A full cafeteria open from 6:30 a.m. - 2:30 p.m. and a concession stand open from 7:00 a.m. - 3:30 p.m. are located on the main level of Natcher.

For your convienence, pre-ordered box lunches can be purchased from the cafeteria and delivered to the meeting during the lunch break/poster session on April 20. To order, select your meal from the menu and follow the posted instructions on how to place your order.

LODGING INFORMATION

With the exception of invited keynote speakers, all other participants and presenters are responsible for all lodging charges, taxes, and incidentals.

Hyatt Regency Bethesda (1.5 miles from NIH)

Address: One Bethesda Metro Center (7400 Wisconsin Ave), Bethesda, Maryland, 20814
Phone: (301) 657-1234
Website: bethesda.hyatt.com

Located in the heart of downtown Bethesda, Maryland, Hyatt Regency Bethesda is surrounded by numerous shopping and dining options. The Washington Metro Red Line (Bethesda Station) is situated directly below the hotel and is one stop away from the NIH (Medical Center Station).

A block of rooms has been reserved for the meeting at $229.00 per night + 13% tax (single occupancy; other options available). Rate expires on March 19 or when room limit reached. Please contact the hotel directly regarding any reservation changes or questions.

To book a room online, click here.

To book a room via phone, call 1-888-421-1442 and reference "Single Cell Analysis".

FAQs

Can I update my registration information?

Who should I contact with questions?

  • For questions about meeting content, logistics, registration changes, or abstract submissions, please contact Cathy Ng (NIH\NIMH) and Becky Miller (NIH\OD) at single_cell@mail.nih.gov.

FAQs FOR PRESENTERS

Should all SCAP projects present?

  • Yes. Each group with an active SCAP award must prepare a poster or apply for a talk (limited space available) describing the funded single cell analysis project and current progress.
  • Posters from FY14 SCAP grants should address the goals, proposed methods, expected outcomes, and supporting pilot data.
  • Designate one individual as the primary presenter of the poster for each SCAP award.

Do I need to submit an abstract?

  • If you wish to present a poster or talk at the meeting, you must submit an abstract.
  • Investigators actively funded under SCAP RFAs are required to submit an abstract describing their work and progress. Due to a limited number of slots for both presentations and posters, we ask for one abstract per award. Please coordinate among your award collaborators to determine the abstract you wish to submit.
  • Funded investigators who have not spoken at previous meetings will be given priority during talk selection, but everyone is welcome to submit an abstract for either format.

How do I submit an abstract?

  • If you are interested in presenting a talk or a poster, please indicate your interest during registration, format your abstract following the Abstract Template guidelines, and email your abstract to single_cell@mail.nih.gov. ALL abstracts are due by Friday, March 27, 2015.
    • If you are an FY14 SCAP awardee, we will use the title and abstract you submitted with your FY14 application. You do not need to write a new abstract.
    • Please include relevant NIH grant numbers in your acknowledgements section.

When will my abstract be approved?

  • You will receive a confirmation email when your abstract is submitted and a notification email when your abstract has been selected for a presentation or poster. All SCAP grantees are guaranteed poster space.

What are the presentation or poster requirements?

  • Posters should be no more than 4 ft x 4 ft in size. Thumbtacks/velcro will be provided on-site. Each presenter will receive an assigned number and designated space for the poster session on Monday, April 20th.

USEFUL DOCUMENTS

To download any of these files, right click the hyperlink and choose "Save Link As". Hard copies of the Agenda and Poster List will be available at the meeting.

Do NOT use Internet Explorer when trying to access these documents; use an alternate browser.

Materials from previous meetings are posted on the Single Cell Analysis Program Website.


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